YSI TruLine Ammonia Electrode Manual

YSI Ikke kategoriseret TruLine Ammonia Electrode

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Side 1/2

Required Equipment

• Ion meter (preferred) or pH/mV meter.

• Wash bottle with distilled or deionized water.

• . Several clean beakers (glass, 150 mL preferred)

• . Pipette for measuring 10 mL or less

• Graduated cylinder (100 mL)

• * Fill Solution YSI #400367

• ( ) 1000 mg/L ppm Ammonia Standard YSI #400370*

• ) 100 mg/L (ppm Ammonia Standard #400369 YSI

• Ionic Strength Adjustor (ISA) YSI #400368*

• Replacement Membrane Modules (pk/4) YSI #400366**

*Small bottles included with ammonia kit (YSI #400365)ISE

**Two Membrane modules included with ammonia ISE kit.

Overview

The 400365 YSI a - Ammonia Ion Selective Electrode is gas

sensing electrode for measuring ammonia (NH

) in aqueous

samples. be used that buffers sample It must with an ISA the

>pH 11 to cause the ammonia in the sample to become

gaseous. Ammonia Electrode behind an The a ISE is pH

ammonia gas permeable – membrane the ammonia gas

causes pH shift that correlated a in the Fill Solution is to

ammonia concentration based on the result of calibrations .

• Technology: Gas sensing technology

• Size and material:

Membrane Body — (ABS) 12 mm OD x 158 L mm

Cap — x (ABS) 16 mm OD 37 L mm

Cable (Coax 00cm – 1type) ; BNC Connector

• a -Reference: The pH electrode inside has single

junction, Ag/AgCl, annular ceramic junction.

• - Features: Replaceable, pre formed membrane module.

The a pH ISA has indicator that indicates the pH is in

range by a (>11) the presence of blue coloration.

• Range: 0.02- 17,000 mg/L

• Reproducibility: ±2%

• @ Slope: 54 to 59 mv/decade 25°C typical

• pH range: >11

• 0 Operating Temperature: to 50°C

• Interferences: Volatile amines

• Applications/Notes: Popular for wastewater,

aquaculture, agriculture, and industrial applications.

Electrode Preparation

1. The glass pH sensor and reference junction of the pH

electrode should be kept conditioned during transit and

storage. the of the solutionUnscrew cap storage bottle

(Soaker and electrode. Bottle) remove the pH

2. Fill a Membrane Module with Fill Solution

a) Add - s 15 20 drop of Fill Solution to the Membrane Module

b) Tap Module ensure bubbles the Membrane to no are

present . in the fill solution

c) Thread Membrane Module the the slowly into Electrode

Cap. If the Module was overfilled, the excess Fill Solution

will leak out. Avoid rupturing the Membrane Module with

too much Fill Solution.

3. Preferably, allow the pH electrode to condition within the

filled 2 Membrane Module for hours prior to use. The

Membrane Module can be conditioned by placing in a low

to mid-range standard for at least 15 minutes prior to use.

Calibration

1. Connect the assembled ammonia electrode to a meter with

BNC input. Meters with an ISE mode can directly display ion

concentration (e.g. display in mg/L). Ensure the meter is

setup for ammonia and be sure to follow the calibration

procedure in the meter user manual.

Alternatively, a meter with a mV mode can be used, although

the instrument will not display the result of calibration (i.e.

calibration curve will need to be created by the user).

2. For highest accuracy, use a meter with automatic

temperature compensation (ATC) and connect a temperature

sensor.

3. Prepare Standards:

At least 2 standards that bracket the expected sample

range must be used. As an example, prepare 1 mg/L and

10 mg/L standards if the expected sample is ~5 mg/L.

Some meters allow up to a 7 point calibration.

b) There must be at least one order of magnitude

difference between high and low standards (e.g. 10

mg/L and 100 mg/L).

c) If standards span more than one order of magnitude

(e.g. 1 mg/L and 100 mg/L), it is best to prepare at least

one mid-range standard (e.g. 10 mg/L).

d) Each standard should have a volume of 100 mL and

should be placed in a 150 mL glass beaker.

e) Standards should be fresh and carefully prepared. It is

best to use a pipette when measuring small volumes

(10 mL or less) of a more concentrated standard.

f) Use the equation C1*V1=C2*V2 when preparing diluted

standards from a more concentrated standard solution.

As an example, if using the YSI 100 mg/L standard as a

stock solution (YSI #400369) to make 100 mL of a 10

mg/L standard: C1=100 mg/L, V1=?, C2=10 mg/L, and

V2=100 mL. V1=(C2*V2)/C1 =10 mL.

Therefore, 10 mL of the 100 mg/L stock standard should

be added to 90 mL of DI water (i.e. solution should be 100

mL) to make a 10 mg/L standard.

Note: It is not recommended to use a very concentrated

standard (e.g. 1000 mg/L) to make a very dilute standard

(e.g. 0.1 mg/L).

4. Place the electrode in the lowest concentration standard and

stir at a constant rate using a stir bar and stir plate, although

the stirring speed should be limited to minimize loss of

ammonia gas. Use the same stirring rate throughout

calibration.

If you do not have a stir plate, it is best to swirl the solution

or use a stir rod once ISA is added.

5. Add 2 mL of ammonia ISA to the first standard:

a) Using a pipette is recommended. The standard solution

should have a pale blue color that persists after ISA is

added.

b) Allow the solution to stir for 1 minute and begin

calibration. Calibration is time sensitive, as ammonia

diffuses from the sample ~4 minutes after ISA is added.

Therefore, ensure calibration with the standard is

complete 1 to 4 minutes after ISA is added. This timing

is critical! If calibration using the standard is

unsuccessful within this time limit, a fresh standard will

n eed to be prepared.

6. After calibrating with the first point, finish calibrating using

steps #4 and #5 for the remaining calibration points.

C alibrate in order of increasing concentration.

7. Evaluate electrode slope:

a) View the calibration record on the ISE meter, or create

a curve if using a meter with only a mV mode.

b) For highest accuracy, the ammonia electrode slope

should be between 52 mV/decade and - -60 mV/decade.

8. Recalibrate often:

a) The ammonia electrode should be calibrated at the

beginning of each day.

b) For best accuracy, it is best to calibrate the ammonia

electrode every 10 samples. If this calibration interval is

too frequent, at least verify your calibration result every

2 hours by preparing a fresh low to mid range standard, -

adding ISA, and verifying the reading. If the mV reading

has changed ~3 mV compared to the reading in that

standard during calibration, you will need to recalibrate

the electrode.

Measurement

Samples must be prepared like standards. Follow steps #3-5

of the calibration procedure when preparing samples and

taking a measurement.

In summary, 100 mL of sample should be collected and 2 mL

of ISA added. The sample should be fresh and the same

stirring rate using during calibration should be utilized. A

measurement can be taken 1 to 4 minutes after ISA is added.

The temperature of measurement solutions should be as

close as possible to solution temperatures during calibration.

Produkt Specifikationer

Mærke:	YSI
Kategori:	Ikke kategoriseret
Model:	TruLine Ammonia Electrode

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