2
4.2 Aerosol Particle Size Distribution Testing(By Marple 298 Cascade Impactor)
4.2.1 Each sample should be tested with 2.5 ml 0.9% saline solution.
4.2.2 Add 2.5 ml 0.9% saline solution into the nebulizer kit, measure the weight before
and after the testing.
4.2.3 Connect suction and sampling pumps to the cascade impactor testing module as
see in the Fig 2.
4.2.4 Connect the nebulizer kit with NB80 and connect the nebulizer kit outlet to
cascade impactor inlet.(Fig 2.)
4.2.5 The suction and sampling pumps are turned on and allowed to stabilize at
required flows.
4.2.6 Finally start the NB80.(Sampling times can be varied for different nebulizers to
allow for maximum deposit on each stage without coverloading stages.
4.2.7 After sampling for the required time, NB80 is switched off, followed a few
seconds later by the sampling pump and then the suction pump.
4.2.8 Dismount the cascaade impactor from the testing module
4.2.9 Dismantle the impactor and determine the amount of NaCl on the individual
stages of the impactor, the input connection and the outlet filter.
Fig 2. Cascade Impactor and Testing setup
4.3 Nebulization Rate Testing(Including Drug Testings)
4.3.1 Each sample should be tested with 2.0 ml 0.9% saline solution, Atrovent
Ipratropium Bromide, Atrovent Flixotide, AstraZeneca Terbutaline Sulphate and
Ventoline (2.5mg) Salbutamol/Sulphate for 1 minutes.
4.3.2 Add 2.0ml 0.9% saline solution/drugs into the nebulizer kit, measure the weight
before and after the testing.
4.3.3 Connect the nebulizer kit with NB80 and then start NB80 for testing.
4.3.4 After 1.0 minutes have been reached, stop the NB80
4.3.5 Calculates how many weight of the solution/drugs have been nebulizered
4.4 Residual Volume Testing
4.4.1 Each sample should be tested with 2.0ml 0.9% saline solution and nebulized till
the bottle is empty.
4.4.2 Add 2.0ml 0.9% saline solution into the nebulizer kit, measure the weight before
and after the testing.
